Composite

Part:BBa_K2765050:Design

Designed by: Fa He   Group: iGEM18_BIT-China   (2018-10-08)


Complete roGFP2-Orp1 expression cassette started by the promoter TEF2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1252
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1564


Design Notes

NO


Source

Connected pTEF2 and roGFP2-Orp1 by OE-PCR. And ligated the fragment to the plasmid pESC, and pTEF2-roGFP2-Orp1-CYC1t was obtained by PCR.

References